mouse anti rat stro 1  (R&D Systems)

Journal: Archives of oral biology

Article Title: Identification and Characterization of Neural Crest-derived Cells in Adult Periodontal Ligament of Mice

doi: 10.1016/j.archoralbio.2012.04.022

Figure Lengend Snippet: Primary and secondary antibodies used in this study.

Article Snippet: Hematoxylin was used for counter-staining. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Name Clone No. Manufacturer/Distributer Dilution rabbit anti-GFP {"type":"entrez-nucleotide","attrs":{"text":"A11122","term_id":"490966","term_text":"A11122"}} A11122 Molecular Probes (Carlsbad, CA) 1:100 chicken anti-GFP ab13970 abcam (Cambridge, MA) 1:200 biotinylated anti-β-galactosidase G1041-42 US Biological (Massachusetts, MA) 1:100 mouse anti-Slug 62.1E6 DSHB 1:100 mouse anti-AP-2 alpha 3B5 DSHB 1:100 mouse anti-Nestin Rat-401 DSHB 1:100 mouse anti-STRO-1 STRO-1 DSHB 1:100 mouse anti-HNK-1 VC1.1 Sigma-Aldrich (St. Louis, MO) 1:100 mouse anti-p75 ME20.4 Santa Cruz Biotechnology, Inc. (Santa Cruz, CA) 1:50 mouse anti-CD29 18/CD29 BD Bioscience (Franklin Lakes, NJ) 1:100 rabbit anti-phospho-histone H3 Ser10 Millipore (Temecula, CA) 1:100 Alexa Fluor 488 anti-rabbit IgG n/a Molecular Probes (Carlsbad, CA) 1:100 Alexa Fluor 488 anti-chicken IgG n/a Molecular Probes (Carlsbad, CA) 1:100 Alexa Fluor 594 anti-mouse IgG 1 n/a Molecular Probes (Carlsbad, CA) 1:100 Alexa Fluor 568 anti-mouse IgG 2b n/a Molecular Probes (Carlsbad, CA) 1:100 Alexa Fluor 568 anti-mouse IgM n/a Molecular Probes (Carlsbad, CA) 1:100 biotinylated anti-rabbit IgG n/a Vector Laboratories (Burlingame, CA) 1:200 biotinylated anti-chiken IgG n/a Vector Laboratories (Burlingame, CA) 1:200 Open in a separate window DSHB; Developmental Studies Hybridoma Bank, Department of Biology, The University of Iowa Primary and secondary antibodies used in this study.

Techniques: Plasmid Preparation

Journal: Archives of oral biology

Article Title: Identification and Characterization of Neural Crest-derived Cells in Adult Periodontal Ligament of Mice

doi: 10.1016/j.archoralbio.2012.04.022

Figure Lengend Snippet: Immunofluorescence staining of CD29 and STRO-1 on PDL cells (a). CD29 or STRO-1(+) cells were found only on GFP(−) cells and showed perivascular localization (arrowheads). Red blood cells are detected due to their autofluorescence property (open arrowheads). The expression of phospho-Histone H3 (pHH3)(+) cells among GFP(+) NCCs and GFP(−) cells were shown (b). GFP(+) cells showed significantly higher cell proliferation in comparison to GFP(−) cells. Bar: 20 µm. *: Statistical significance p<0.05.

Article Snippet: Hematoxylin was used for counter-staining. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Name Clone No. Manufacturer/Distributer Dilution rabbit anti-GFP {"type":"entrez-nucleotide","attrs":{"text":"A11122","term_id":"490966","term_text":"A11122"}} A11122 Molecular Probes (Carlsbad, CA) 1:100 chicken anti-GFP ab13970 abcam (Cambridge, MA) 1:200 biotinylated anti-β-galactosidase G1041-42 US Biological (Massachusetts, MA) 1:100 mouse anti-Slug 62.1E6 DSHB 1:100 mouse anti-AP-2 alpha 3B5 DSHB 1:100 mouse anti-Nestin Rat-401 DSHB 1:100 mouse anti-STRO-1 STRO-1 DSHB 1:100 mouse anti-HNK-1 VC1.1 Sigma-Aldrich (St. Louis, MO) 1:100 mouse anti-p75 ME20.4 Santa Cruz Biotechnology, Inc. (Santa Cruz, CA) 1:50 mouse anti-CD29 18/CD29 BD Bioscience (Franklin Lakes, NJ) 1:100 rabbit anti-phospho-histone H3 Ser10 Millipore (Temecula, CA) 1:100 Alexa Fluor 488 anti-rabbit IgG n/a Molecular Probes (Carlsbad, CA) 1:100 Alexa Fluor 488 anti-chicken IgG n/a Molecular Probes (Carlsbad, CA) 1:100 Alexa Fluor 594 anti-mouse IgG 1 n/a Molecular Probes (Carlsbad, CA) 1:100 Alexa Fluor 568 anti-mouse IgG 2b n/a Molecular Probes (Carlsbad, CA) 1:100 Alexa Fluor 568 anti-mouse IgM n/a Molecular Probes (Carlsbad, CA) 1:100 biotinylated anti-rabbit IgG n/a Vector Laboratories (Burlingame, CA) 1:200 biotinylated anti-chiken IgG n/a Vector Laboratories (Burlingame, CA) 1:200 Open in a separate window DSHB; Developmental Studies Hybridoma Bank, Department of Biology, The University of Iowa Primary and secondary antibodies used in this study.

Techniques: Immunofluorescence, Staining, Expressing